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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
29/09/2014 |
Actualizado : |
24/06/2021 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
FEDERICI, M.; MARCONDES, J.A.; PICCHI, S.C.; STUCHI, E.S.; FADEL, A.L.; LAIA, M.L.; LEMOS, M.V.F. |
Afiliación : |
MARIA TERESA FEDERICI RODRIGUEZ, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
Título : |
Xylella fastidiosa: An in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis. |
Fecha de publicación : |
2012 |
Fuente / Imprenta : |
Electronic Journal of Biotechnology, 2012, v.15, no.3, p.1-33. |
ISSN : |
0717-3458 |
DOI : |
http://dx.doi.org/10.2225/vol15-issue3-fulltext-4 |
Idioma : |
Inglés |
Contenido : |
ABSTRACT.
Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increase insight into stress responses and possible bacterial survival mechanisms in the nutrient-poor environment of xylem vessels. MenosABSTRACT.
Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped... Presentar Todo |
Thesagro : |
CITRUS; CYDIA. |
Asunto categoría : |
F01 Cultivo |
Marc : |
LEADER 02405naa a2200241 a 4500 001 1050705 005 2021-06-24 008 2012 bl uuuu u00u1 u #d 022 $a0717-3458 024 7 $ahttp://dx.doi.org/10.2225/vol15-issue3-fulltext-4$2DOI 100 1 $aFEDERICI, M. 245 $aXylella fastidiosa$bAn in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis.$h[electronic resource] 260 $c2012 520 $aABSTRACT. Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increase insight into stress responses and possible bacterial survival mechanisms in the nutrient-poor environment of xylem vessels. 650 $aCITRUS 650 $aCYDIA 700 1 $aMARCONDES, J.A. 700 1 $aPICCHI, S.C. 700 1 $aSTUCHI, E.S. 700 1 $aFADEL, A.L. 700 1 $aLAIA, M.L. 700 1 $aLEMOS, M.V.F. 773 $tElectronic Journal of Biotechnology, 2012$gv.15, no.3, p.1-33.
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INIA Las Brujas (LB) |
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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
14/09/2022 |
Actualizado : |
14/09/2022 |
Tipo de producción científica : |
Abstracts/Resúmenes |
Autor : |
DE BARBIERI, I.; FERREIRA, G.; RAMOS, Z.; NAVAJAS, E.; CIAPPESONI, G. |
Afiliación : |
LUIS IGNACIO DE BARBIERI ETCHEBERRY, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GUSTAVO ALVARO FERREIRA DE MATTOS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ZULLY MARGOT RAMOS ALVEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ELLY ANA NAVAJAS VALENTINI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS GABRIEL CIAPPESONI SCARONE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Consequences of contrasting feed efficiency as lamb on later ewe performance. [Abstract] |
Fecha de publicación : |
2022 |
Fuente / Imprenta : |
In: Annual Meeting of the European Federation of Animal Science (EAAP), 73., Porto, Portugal, 5-8 September 2022. Book of Abstracts, 73., Session 68, Theatre 4, p.674. |
ISBN : |
978-90-8686-937-4; e-ISBN: 978-90-8686-385-3 |
Idioma : |
Inglés |
Notas : |
Conference website: https://eaap2022.org/ |
Contenido : |
The aim of this study was to evaluate the productive and reproductive performance of ewes with contrasting residual feed intake (RFI) measured as lamb in their first year of life (in the frame of H2020 project SMARTER n°772787). |
Palabras claves : |
EWE; Proyecto SMARTER; Residual feed intake (RFI); SHEEP. |
Asunto categoría : |
L01 Ganadería |
Marc : |
LEADER 01008nam a2200217 a 4500 001 1063569 005 2022-09-14 008 2022 bl uuuu u01u1 u #d 100 1 $aDE BARBIERI, I. 245 $aConsequences of contrasting feed efficiency as lamb on later ewe performance. [Abstract]$h[electronic resource] 260 $aIn: Annual Meeting of the European Federation of Animal Science (EAAP), 73., Porto, Portugal, 5-8 September 2022. Book of Abstracts, 73., Session 68, Theatre 4, p.674.$c2022 500 $aConference website: https://eaap2022.org/ 520 $aThe aim of this study was to evaluate the productive and reproductive performance of ewes with contrasting residual feed intake (RFI) measured as lamb in their first year of life (in the frame of H2020 project SMARTER n°772787). 653 $aEWE 653 $aProyecto SMARTER 653 $aResidual feed intake (RFI) 653 $aSHEEP 700 1 $aFERREIRA, G. 700 1 $aRAMOS, Z. 700 1 $aNAVAJAS, E. 700 1 $aCIAPPESONI, G.
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